Analysis Note

Positive ControlLysates from COS cells treated with UV

Application

Immunoblotting (1:1000)Immunocytochemistry (1:500, fluorescence)Paraffin Sections (1:50)

General description

Recognizes the ~56 kDa Chk1 protein phosphorylated at Ser³¹⁷ in UV-treated COS cells.

This PhosphoDetect™ Anti-Chk1 (pSer³¹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of Chk1 (pSer³¹⁷).

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~56 kDa Chk1 protein when phosphorylated at Ser³¹⁷.

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³¹⁷ phosphorylation site of human Chk1

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Does not recognize unphosphorylated Chk1. Antibody should be titrated for optimal results in individual systems.

Zhao, H. and Piwnica-Worms, H. 2001. Mol. Cell. Biol.21, 4129.Shieh, S.Y., et al. 2000. Genes Dev.14, 289.Martinho, R.G., et al. 1998. EMBO J.17, 7239.Zeng, Y., et al. 1998. Nature395, 507.

Packaging

50 µL in Plastic ampoule

Physical form

In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Reconstitution

Do not aliquot.

Warning

Toxicity: Standard Handling (A)